Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon

• DOI: 10.1016/j.jot.2023.10.007
• Publication Date: 2024-03-13T11:16:36Z
• Authors: Guidong Shi; Nakagawa Koichi; Rou Wan; Yicun Wang; Ramona Reisdorf; Abigayle Wilson; Tony C.T. Huang; Peter C. Amadio; Alexander Meves; Chunfeng Zhao; Steven L. Moran
• Keywords: Pentamidine; Gelatin; Viability assay

Prevention of adhesion formation following flexor tendon repair is essential for restoration normal finger function. Although many medications have been studied in the experimental setting to prevent adhesions, clinical application limited due complexity and delivery translation. In this study, optimal dosages gelatin pentamidine were validated by concentration test. Following cell viability, migration, live dead cell, assay Turkey tenocytes, a model was established evaluate effectiveness Pentamidine-Gelatin sheet. Pentamidine carried with gelatin, Food drug administration (FDA) approved material delivery, showed good dynamic release, biocompatibility, degradation. The dose (25ug) determined vivo study using tenocyte assays. Further biochemical analyses demonstrated that positive effect may be downregulating Wnt signaling pathway without affecting collagen expression. We tested FDA-approved antibiotic, pentamidine, reducing after both vitro novel turkey animal model. Compared non-pentamidine treatment group, treated turkeys had significantly reduced adhesions improved digit function six weeks healing. This first time common drug, has potential reduce improve gliding interfering