Methylglyoxal-induced stomatal closure accompanied by peroxidase-mediated ROS production in Arabidopsis

• DOI: 10.1016/j.jplph.2012.02.007
• Publication Date: 2012-03-19T12:46:22Z
• Authors: Tahsina Sharmin H...; Misugi Uraji; Wenxiu Ye; Mohammad Anowar H...; Yoshimasa Nakamura; Yoshiyuki Murata
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Cytosol; Mutation; Plant Stomata; Arabidopsis; Calcium Signaling; Pyruvaldehyde; Reactive Oxygen Species; Peroxidase

Methylglyoxal (MG) is an oxygenated short aldehyde and a glycolytic intermediate that accumulates in plants under environmental stresses. Being a reactive α-oxoaldehyde, MG may act as a signaling molecule in plants during stresses. We investigated whether MG induces stomatal closure, reactive oxygen species (ROS) production, and cytosolic free calcium concentration ([Ca²⁺](cyt)) to clarify roles of MG in Arabidopsis guard cells. MG induced production of ROS and [Ca²⁺](cyt) oscillations, leading to stomatal closure. The MG-induced stomatal closure and ROS production were completely inhibited by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), but were not affected by an NAD(P)H oxidase mutation, atrbohD atrbohF. Furthermore, the MG-elicited [Ca²⁺](cyt) oscillations were significantly suppressed by SHAM but not by the atrbohD atrbohF mutation. Neither endogenous abscisic acid nor endogenous methyl jasmonate was involved in MG-induced stomatal closure. These results suggest that intrinsic metabolite MG can induce stomatal closure in Arabidopsis accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca²⁺](cyt) oscillations.