Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay

• DOI: 10.1016/j.jprot.2021.104355
• Publication Date: 2021-08-24T16:09:00Z
• Authors: Simon Ngao Mule; Livia Rosa-Fernandes; João V.P. Coutinho; Vinícius De Morai...; Janaina Macedo-da...; Verônica Feijoli ...; Daniel Quina; Gilberto Santos d...; Morten Thaysen-An...; Martin R. Larsen; Letícia Labriola; Giuseppe Palmisano
• Keywords: PNGase F

AbstractA new method to probe the conformational changes of glycoproteins on a systems-wide scale, termed limited deglycosylation assay (LDA), is described. The method measures the differential rate of deglycosylation of N-glycans on natively folded proteins by the common peptide:N-glycosidase F (PNGase F) enzyme which in turn informs on their spatial presentation and solvent exposure on the protein surface hence ultimately the glycoprotein conformation. LDA involves 1) protein-level N-deglycosylation under native conditions, 2) trypsin digestion under denaturing conditions, 3) glycopeptide enrichment, 4) peptide-level N-deglycosylation and 5) quantitative MS-based analysis of the formerly N-glycosylated peptides. LDA was initially developed and the experimental conditions optimized using bovine RNase B and fetuin. The method was then applied to glycoprotein extracts from LLC-MK2 epithelial cells upon treatment with dithiothreitol to induce endoplasmic reticulum stress and promote protein misfolding. Data from the LDA and 3D structure analysis showed that glycoproteins predominantly undergo structural changes in loops/turns upon ER stress as exemplified with detailed analysis of ephrin-A5, GALNT10, PVR and BCAM. These results show that LDA accurately reports on systems-wide conformational changes of glycoproteins induced under controlled treatment regimes. Thus, LDA opens avenues to study glycoprotein structural changes in a range of other physiological and pathophysiological conditions relevant to acute and chronic diseases.