4Pi-microscopy of the Golgi apparatus in live mammalian cells

• DOI: 10.1016/j.jsb.2003.10.006
• Publication Date: 2003-11-06T13:02:59Z
• Authors: Alexander Egner; Sophie Verrier; Alexander Goroshkov; Hans-Dieter Söling; Stefan W. Hell
• Keywords: Live cell imaging; Interference microscopy

We report the applicability of 4Pi-microscopy to live mammalian cells. Controlled interference of the counterpropagating wavefronts is possible despite the slight variations in cellular refractive index. Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at approximately 100 nm resolution.