Effect of cryoprotection on particle size stability and preservation of chitosan nanoparticles with and without hyaluronate or alginate coating

• DOI: 10.1016/j.jsps.2016.12.008
• Publication Date: 2017-01-10T18:31:41Z
• Authors: Abdulaziz Almalik; Ibrahim Alradwan; Mohd Abul Kalam; Aws Alshamsan
• Keywords: Cryoprotectant; Alginic acid

The aim of the present study was to determine effect different cryoprotectants and their concentration on physicochemical characteristics chitosan nanoparticles (CS-NPs). coating CS-NPs with hyaluronic acid (HA) alginic (ALG) before after lyophilization also evaluated. ionic gelation method used for preparation NPs six types (sucrose, glucose, trehalose, mannitol, polyethylene glycol-2000, glycol-10,000) were investigated at 5%, 10%, 20%, 50% levels. Coating HA protection high amount indicated better particle size stability. Samples that lyophilized without resulted in an increase average due agglomeration. All varying provided some sort stability except PEG-10,000 which had no protective higher concentrations. Sucrose trehalose sugars found have highest coated uncoated CS-NPs. In conclusion, using along surface coating, could achieve desired a prolonged duration.