Evaluation of microalgae and cyanobacteria as potential sources of antimicrobial compounds

• DOI: 10.1016/j.jsps.2020.11.010
• Publication Date: 2020-11-26T01:15:16Z
• Authors: Faisal Alsenani; Karnaker R. Tupally; Elvis T. Chua; Eladl Eltanahy; Hamed Alsufyani; Harendra S. Parekh; Peer M. Schenk
• Keywords: Bioactive compound

In recent decades, marine microorganisms have become known for their ability to produce a wide variety of secondary bioactive metabolites. Several compounds been isolated from the development novel bioactives food and pharmaceutical industries. this study, number microalgae were evaluated antimicrobial activity against gram-positive gram-negative bacteria, including plant pathogens, using various extraction techniques assays. Disc diffusion spot-on-lawn assays conducted confirm activity. To measure potency extracts, minimum inhibition concentrations (MIultCs) measured. Three microalgae, namely Isochrysis galbana, Scenedesmus sp. NT8c, Chlorella FN1, showed strong inhibitory preferentially bacteria. These microalgal species then selected further purification analysis, leading compound identification. By mixture different chromatography gas chromatography-mass spectrometry (GC-MS) high-performance liquid (HPLC) ultra-high performance chromatography-quadrupole time-of-flight mass (UHPLC-Q-TOF-MS), we able separate identify dominant that are responsible Additionally, nuclear magnetic resonance (NMR) was used presence these compounds. The identified purified in extracts linoleic acid, oleic docosahexaenoic acid (DHA) eicosapentaenoic (EPA). potential candidates inhibit growth This indicates use as biocontrol agents pathogens.