Design, physicochemical characterisation, and in vitro cytotoxicity of cisplatin-loaded PEGylated chitosan injectable nano / sub-micron crystals

• DOI: 10.1016/j.jsps.2023.04.005
• Publication Date: 2023-04-13T09:18:51Z
• Authors: Muhammad H. Sultan; Sivakumar S. Moni; Saad S. Alqahtani; Mohammed Ali Bakkari; Abdulrahman Alsha...; Yosif Almoshari; Saeed Alshahrani; Osama A. Madkhali; Syam Mohan
• Keywords: Zeta potential; Thermogravimetric analysis

The study aimed to develop cisplatin-loaded PEGylated chitosan nanoparticles. optimal batch of nanoparticles had a + 49.9 mV zeta potential, PDI 0.347, and % 58.9. Nanoparticle size was 741.4 z. d.nm, the in diameter 866.7 ± 470.5 nm, nanoparticle conductivity colloidal solution 0.739 mS/cm. Differential scanning calorimetry (DSC) revealed that sharp endothermic peaks at temperatures 168.6 °C. thermogravimetric analysis (TGA) showed weight loss nanoparticles, which observed as 95% 262.76 XRD investigation on exhibited distinct 2θ 9.7°, 20.4°, 22.1°, 25.3°, 36.1°, 38.1°, 39.5°, 44.3°, 64.5°, confirming crystalline structure. 1H NMR fingerprint region 0.85, 1.73, 1.00 ppm proton dimension de-shielded appeared 3.57, 3.58, 3.59, 3.65, 3.67, 3,67, 3.70, 3.71, 3.77, 3.78 4.71 ppm. 13C spectrum specified 63.18, 69.20, 70.77 FT-IR spectra cisplatin loaded show existence many regions 3186.52, 2931.68, 1453.19, 1333.98, 1253.71, 1085.19, 1019.60, 969.98, 929.53, 888.80, 706.13, 623.67 cm-1. drug release kinetics zero order with 48% linearity fashion has R2 value 0.9778. Studies MCF-7 ATCC human breast cancer cell line vitro IC50 82.08 µg /mL. Injectable good physicochemical cytotoxic properties. This method is novel since application PEGylation processes leads an increased solubility near neutral pH.