Heat stress inhibits expression of the cytokines, and NF-κB-NLRP3 signaling pathway in broiler chickens infected with salmonella typhimurium

• DOI: 10.1016/j.jtherbio.2021.102945
• Publication Date: 2021-04-15T04:47:22Z
• Authors: Lu-Ping Tang; Wei-Hao Li; Yi-Lei Liu; Jian-Chi Lun; Yong-Ming He
• Keywords: Proinflammatory cytokine

High ambient temperature has potential influence on oxidative stress, or systemic inflammation affecting poultry production and immune status of chickens. Heat stress (HS) induces intestinal increases susceptibility harmful pathogens, such as Salmonella Escherichia coli. Intestinal is a common result body dysfunction. Therefore, we designed an experiment to analyze the effects 35 ± 2 °C HS salmonella infection in chickens through regulation responses. 40 broiler were randomly divided into 4 groups: control group, heat typhimurium (ST) group model (heat + typhimurium, ST). Birds treated with 6 h day for 14 continuous days. Then, ST birds orally administrated 1 mL inoculum (109 cfu/mL). Chickens sacrificed at 4th after administration ileum tissues measured. We observed that decreased TNF-α IL-1β protein expressions. Concomitantly NLRP3 Caspase-1 levels. The expressions p-NF-κB-p65 p-IκB-α ileum. also inhibited IFN-α, p-IRF3 p-TBK1, showing deficiency birds. Together, present data suggested suppressed activity infected by decrease cytokines levels, which regulated NF-κB-NLRP3 signaling pathway.