The cell membrane repair protein MG53 modulates transcription factor NF-κB signaling to control kidney fibrosis

• DOI: 10.1016/j.kint.2021.09.027
• Publication Date: 2021-10-30T02:36:53Z
• Authors: Haichang Li; Pu Duann; Zhongguang Li; Xinyu Zhou; Jianjie Ma; Brad H. Rovin; Pei-Hui Lin
• Keywords: 0301 basic medicine; Mice; 0303 health sciences; 03 medical and health sciences; Cell Membrane; NF-kappa B; Animals; Membrane Proteins; Acute Kidney Injury; Kidney; Fibrosis; Ureteral Obstruction; 3. Good health

Kidney fibrosis is associated with the progression of acute kidney injury to chronic disease. MG53, a cell membrane repair protein, has been shown protect against epithelial cells and injury. Here, we evaluated role MG53 in modulation aging mice unilateral ureteral obstruction (UUO) known model progressive fibrosis. Mice ablation developed more interstitial age than MG53-intact same age. Similarly, absence was exaggerated compared intact obstructed contralateral unobstructed or kidneys sham operated mice. The from deficient also showed significantly inflammation In vitro experiments demonstrated that could enter nuclei proximal tubular directly interact p65 component transcription factor NF-κB, providing possible explanation enhanced MG53. To test this, expression through engineered direct recombinant protein delivery given subject UUO. This reduced NF-κB activation attenuated Thus, may have therapeutic treating thereby provide protection leads disease phenotype.