Gene expression profile of endothelial cells exposed to estrogenic environmental compounds: Implications to pulmonary vascular lesions

• DOI: 10.1016/j.lfs.2010.04.007
• Publication Date: 2010-04-24T08:24:46Z
• Authors: Quentin Felty; Changwon Yoo; Amy Kennedy
• Keywords: 0301 basic medicine; 0303 health sciences; Neovascularization, Pathologic; Gene Expression Profiling; Down-Regulation; Endothelial Cells; Bayes Theorem; Estrogens; Environmental Exposure; Polymerase Chain Reaction; Cell Line; Up-Regulation; 3. Good health; 03 medical and health sciences; Microvessels; Medicine and Health Sciences; Cluster Analysis; Humans; Environmental Pollutants; Gene Regulatory Networks; Lung

The cardiovascular system is an important target of estrogenic compounds. Considering the recent studies that question previously reported cardio-protective effects of estrogen, there is a growing concern that estrogenic environmental compounds may contribute to the pathology of vascular lesion formation.Real-time quantitative PCR was used to monitor the expression of genes involved in vascularization. Using Bayesian network modeling, we determined a gene network that estrogenic chemicals modulate in human vascular endothelial cells.We showed that planar and coplanar polychlorinated biphenyls (PCBs) induce the expression of different genes compared to estradiol. Non-planar PCB congener 153 induced NOTCH3 which is a new finding as well as CCL2 and IL8 similar to what has been reported by other non-planar PCBs in endothelial cells. Our gene network indicated that experimental treatments signal a network containing TGF-beta receptor and NOTCH3; molecules biologically relevant to signaling pulmonary vascular lesions.We report in the present study that exposure of vascular endothelial cells to environmentally relevant concentrations of estrogenic PCBs induce gene networks implicated in the process of inflammation and adhesion. Our data suggest that PCBs can promote vascular lesion formation by activating gene networks involved in endothelial cell adhesion, cell growth, and pro-inflammatory molecules which were different from natural estrogen. Since inflammation and adhesion are a hallmark in the pathology of endothelial cell dysfunction, reconstructing gene networks provide insight into the potential mechanisms that may contribute to the vascular risks associated with estrogenic environmental chemicals.