Micturition dysfunction in four-month old ovariectomized rats: Effects of testosterone replacement

• DOI: 10.1016/j.lfs.2017.05.006
• Publication Date: 2017-05-06T06:32:54Z
• Authors: Sandra M. Bonilla...; Mariana G. de Oli...; Fabiano B. Calmasini; Julio A. Rojas-Mo...; Angelina Zanesco; Edson Antunes
• Keywords: Urination; Dihydrotestosterone

Androgen deficiency has been implicated in urological complications of postmenopausal women. This study examined the effects testosterone replacements on lower urinary tract dysfunction 4-month old ovariectomized (OVX) rats.Sprague-Dawley female rats were OVX bilaterally. Three months later, received single intramuscular injections undecanoate. Cystometric study, and bladder urethra smooth muscle reactivities evaluated.Ovariectomy reduced by 65% (p<0.05) serum levels. Testosterone replacement at 5mg/kg restored hormone levels to baseline, whereas 10mg/kg produced 14-fold higher exhibited significant increases body weight, perigonadal fat blood pressure, uterus but none these parameters changed replacements. micturition characterized basal threshold voiding frequency post-voiding pressure. In addition, contractions induced electrical-field stimulation (EFS) carbachol significantly reduced, angiotensin II-induced urethral increased rats. (but not 5mg/kg) dose fully normalized vivo dysfunction, as well vitro alterations. (10mg/kg) also potentiated relaxations β3-adrenoceptor agonist mirabegron. The protective modified concomitant treatment with aromatase inhibitor letrozole (2.5mg/kg, 4weeks).The improvement suggests that androgen therapy might be therapeutic benefit for associated post-menopause.