Protective effects of epigallocatechin gallate against ischemia reperfusion injury in rat skeletal muscle via activating Nrf2/HO-1 signaling pathway

• DOI: 10.1016/j.lfs.2019.117014
• Publication Date: 2019-11-01T08:46:16Z
• Authors: Yan Zhao; Xinhao Liu; Xuejuan Fu; Zhongyun Mo; Yi Jiang; Yanli Yan
• Keywords: Male; 0303 health sciences; NF-E2-Related Factor 2; Apoptosis; Antioxidants; Catechin; Rats; 3. Good health; Activation, Metabolic; Rats, Sprague-Dawley; Oxidative Stress; 03 medical and health sciences; Reperfusion Injury; Heme Oxygenase (Decyclizing); Animals; Cytokines; Muscle, Skeletal; Signal Transduction

Previous studies have demonstrated that epigallocatechin gallate (EGCG) had certain protective effects on myocardial and renal ischemia reperfusion (I/R) injury. We aimed to research the special effects and underling mechanisms of EGCG on skeletal muscle I/R injury.We established an experimental rat model of I/R skeletal muscle injury and treated with different doses of EGCG. Hematoxylin eosin staining, TUNEL assay, ELISA, qRT-PCR and Western blotting were used to evaluate the effects of EGCG.EGCG significantly improved skeletal muscle function of I/R injury rats. Moreover, EGCG had positive effects on decreasing apoptosis of skeletal muscle tissues, alleviating oxidative stress damage and suppressing the production of inflammatory cytokines. Further, EGCG had positive effects on activating Nrf2/HO-1 signaling pathway.EGCG might be a powerful candidate compound for alleviating I/R injury in rat skeletal muscle.