Internal transcribed spacer sequencing and metatranscriptomics analysis reveal the fungal community composition, diversity related environment variables and roles during serofluid dish fermentation

• DOI: 10.1016/j.lwt.2021.112450
• Publication Date: 2021-09-13T12:28:53Z
• Authors: Zhongkun Zhou; Rentao Zhang; Shujian Hu; Yunhao Ma; Kangjia Du; Mengze Sun; Hao Zhang; Xinrong Jiang; Hongyuan Tu; Peng Chen
• Keywords: 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; 15. Life on land

Serofluid dish is a popular fermented food in northwest China that contains abundant microorganisms. Advances of next-generation sequencing facilitate the elucidation bacterial community composition, yet roles fungi serofluid need further exploration. Here, internal transcribed spacer (ITS) and metatranscriptomics were conducted results showed dominant fungal communities belong to Candida Saccharomyces. Additionally, diversity, including phylogenetic diversity species richness, was largely correlated with pH, total organic carbon (TOC) spatial distance. Multivariate regression tree all-subsets analysis also identified them as strong predictors. Further gene annotations revealed exhibited higher participation carbohydrate metabolism, amino acid translation energy metabolism pathways, sucrose maltose production acetate, fumarate ethanol. Collectively, possess rich are affected by both environmental variation geographical In fermentation process, predominantly participated genetic information processing.