Heptanal inhibits the growth of Aspergillus flavus through disturbance of plasma membrane integrity, mitochondrial function and antioxidant enzyme activity

• DOI: 10.1016/j.lwt.2021.112655
• Publication Date: 2021-10-21T17:53:46Z
• Authors: Sheng-Fa Li; Shuai-Bing Zhang; Yang-Yong Lv; Huan-Chen Zhai; Yuan-Sen Hu; Jing-Ping Cai
• Keywords: Heptanal

Fungal contamination poses a threat to agriculture, food production and human health, safe effective method limit fungal is still needed. In this study, the inhibitory potency of heptanal, natural volatile chemical, against Aspergillus flavus growth was evaluated, its mechanism action explored. The minimum concentrations (MICs) heptanal vapor fumigation liquid contact culture were 0.085 0.8 μL/mL, respectively. A total 79 significant differentially abundant metabolites, including 37 upregulated 42 downregulated ones, identified in A. mycelia after exposure μL/mL for 6 h. Metabolomics analysis revealed that had destructive effects on plasma integrity mitochondrial function mycelia. Biochemical confirmed caused intracellular substance leakage resulted reduced ATP content, reactive oxygen species (ROS) accumulation, lower dehydrogenase ATPase activities. Evaluation control efficacy wheat grain under simulated storage conditions 225.0 μL/L could inhibit 19% moisture. These results provide insights into antifungal as promising bio-preservative.