Potent eradication of mixed-species biofilms using photodynamic inactivation coupled with slightly alkaline electrolyzed water

• DOI: 10.1016/j.lwt.2021.112958
• Publication Date: 2021-12-11T04:26:19Z
• Authors: Lijun Tan; Yong Zhao; Yufeng Li; Zhiyun Peng; Tiantian He; Yang Liu; Qiaohui Zeng; Jing Jing Wang
• Keywords: Viability assay; Shewanella putrefaciens; Extracellular polymeric substance

This study pioneered a novel anti-biofilm technology of photodynamic inactivation (PDI) coupled with slightly alkaline electrolyzed water (SAlEW) as solvent to dissolve photosensitizer, and its potency was verified through monitoring the biomass, cell viability, regrowth capacity spatial structures mixed-species biofilms Vibrio parahaemolyticus Shewanella putrefaciens. The action mechanism clarified by measuring reactive oxygen species (ROS), physicochemical properties photosensitizer sodium copper chlorophyll (SCC). Results showed that SAlEW greatly improved permeability stability SCC, well ROS production, which potently destroyed key chemical composition extracellular polymeric substances (EPS) hence negatively altered biofilms. Moreover, PDI significantly decreased biomass 72.4%, viable cells 3.51 Log CFU/mL viability 85.7% in 150 μM SCC 4.56 J/cm2 radiation, notably, it efficiently eradicated on surfaces fish scales or stainless steel, supported reduced 86% 70%, respectively. Therefore, is promising strategy control contamination food industry.