Establishment of anthocyanin fingerprint in black wolfberry fruit for quality and geographical origin identification

• DOI: 10.1016/j.lwt.2022.113080
• Publication Date: 2022-01-10T04:28:58Z
• Authors: Huan Cheng; Wenyan Wu; Jin Chen; Haibo Pan; Enbo Xu; Shiguo Chen; Xingqian Ye; Jianle Chen
• Keywords: Pectin

Black wolfberries (Lycium ruthenicum Murray) establish various biological activities, owing to anthocyanins, polysaccharides, pectin and so on. However, minor appearance differences are found in black from different geographical origins. Thus, this study, total phenolic content (TPC), flavonoid (TFC) anthocyanin (TAC) were determined four provinces China (Xinjiang, Qinghai, Gansu, Ningxia Provinces) for obtaining a distinguishable chemical index. Compared with TPC TFC, TAC largely was affected by environment conditions, thus, it may be good marker origin identification. Then, profiles analysed ultra-performance liquid chromatography (UPLC) coupled triple quadrupole (triple Q)-time-of-flight mass spectrometry (TOF-MS), principal component analysis (PCA) applied identification based on fingerprints. A of 17 anthocyanins tentatively including two new individual delphinidin-3-rutinoside (cis-p-coumaroyl)-5-O-diglucoside (trans-p-coumaroyl)-5-O-diglucoside. PCA indicated that contents calculated according HPLC data raw both allowed clear separation origins wolfberry fruits, explaining more than 95% information the samples. The present study could provide scientific guidance distinguishing