Separation, identification and docking analysis of xanthine oxidase inhibitory peptides from pacific cod bone-flesh mixture

• DOI: 10.1016/j.lwt.2022.113862
• Publication Date: 2022-08-10T13:08:56Z
• Authors: Qiang Zhao; Ying Meng; Juncai Liu; Zelin Hu; Yutong Du; Jianan Sun; Xiangzhao Mao
• Keywords: Xanthine; Docking (animal)

This study aimed to isolate and investigate five novel xanthine oxidase (XO) inhibitory peptides FF, YF, WPW, WPDARG YNVTGW from alkaline protease hydrolyzate (F0) of Pacific cod processing by-product bone-flesh mixture (CBFM2). F0 was separated by dextran gel chromatography obtain F1, F2, F3, F4 F5 components. The components with the highest XO activity were analyzed identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS), further screened molecular docking Autodock Vina. results showed that component had activity, a weight ranging 200 600 Da. Five obtained IC50 values 0.80 mM, 0.52 1.68 0.40 mM 0.23 respectively. According docking, having at least one aromatic amino acid are more favorable occupy catalytic core XO, reducing its activity. acids Thr1010 Phe914 in active pocket interact peptides. Overall, CBFM have potential be used as natural uric ingredient functional health foods.