Inhibitory effects of piperine and black pepper essential oil on multispecies biofilm formation by Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas aeruginosa

• DOI: 10.1016/j.lwt.2023.114851
• Publication Date: 2023-05-15T14:58:54Z
• Authors: Emanoelli Apareci...; Leonardo Ereno Ta...; Jhennifer Arruda ...; Paulo Henrique Si...; Eduarda de Cássia...; Fábio Sossai Poss...; Maria Olivia Pereira; Juliano Gonçalves...; Luciano dos Santo...
• Keywords: Piperine

This study aimed to evaluate the antimicrobial and antibiofilm activities of black pepper essential oil (BPEO) piperine effect on gene expression in a multispecies biofilm composed Listeria monocytogenes, Salmonella Typhimurium, Pseudomonas aeruginosa polypropylene surface. The minimal inhibitory concentrations BPEO were 100 25 mg/mL, respectively, against this consortium microorganisms. Sessile cell counts 5.35–7.35 log CFU/cm2 varied over time. total population eradicated ranged from 78.9% (5.88 CFU/cm2) 99.8% (4.16 CFU/cm2). Evaluation biofilm-related showed upregulation L. monocytogenes genes agrC (24 72 h), agrD (72 prfA h) downregulation all evaluated S. Typhimurium P. untreated control biofilm. addition resulted agrB h); agfA adrA csgD h). Piperine more effectively controlled