Efficacy and mechanisms of Pseudomonas aeruginosa PAO1 biofilm inactivation using high-power pulsed microwave

• DOI: 10.1016/j.lwt.2024.115982
• Publication Date: 2024-03-19T12:38:43Z
• Authors: Fan Wang; Yaxin Zhang; Anthony Pius Bassey; Maleeka Singh; Yongsheng Zhu; Maria G. Corradini; Xiaozhen Cui; Xiaoqian Zhang; Xiaoli Liu

Foodborne pathogens and biofilm formation pose a critical threat to food safety. Pseudomonas aeruginosa is pathogen of interest in the industry. High-power pulsed microwave (HPPM) non-thermal technology with proven efficacy towards bacterial inactivation. This study investigated inactivation mechanisms performance different HPPM treatments (50, 100, 150, 200, 250 Hz for 3, 5, 7, 9, 11 min, respectively) identify optimum conditions inactivate P. PAO1 biofilms. The results showed significant cell at increasing frequency/time (p < 0.05). Hence, treatment (200 Hz, 9 min) was selected following its limited thermal sublethal effects elucidate further against biofilm-forming ability. Cell viability, scanning (SEM) transmission electron microscopy (TEM) demonstrated severe morphological disruptions, disintegration, lysis treated cells. From confocal laser micrographs (CLSM), thickness calculated, showing reduction after (17.56 vs. 42.16 μm untreated groups, respectively), indicating that effectively compromised integrity envelope. outlines useful insights into better understanding HPPM's on microbial proliferation