The effects of aligned poly(lactic-co-glycolic acid) nanofibrous mat containing gold nanoparticles after planting onto an injured spinal cord

• DOI: 10.1016/j.matdes.2023.112181
• Publication Date: 2023-07-20T02:11:53Z
• Authors: Wan-Kyu Ko; Haram Nah; Seong Jun Kim; Gong Ho Han; Daye Lee; Jae Seo Lee; Dong Nyoung Heo; Donghyun Lee; Sang Jin Lee; Min Heo; Seung Hun Sheen; Il Keun Kwon; Seil Sohn
• Keywords: PLGA; Glycolic acid; Electrospinning

Transplanted cells rarely survive because of the inflammatory cytokines after spinal cord injury. To increase survival, we synthesized a hydrophilic and aligned nanofibrous mat. We electrospun thiolate (SH)/aligned (A) poly(lactic-co-glycolic acid) (PLGA) mats. The PLGA-SH mats were conjugated with gold nanoparticles (GNPs) to hydrophilicity. contact angle is decreased from 105.3° ± 5.2 (for randomly directional PLGA mats) 28.1° 2.8 PLGA-GNP mats). Green fluorescent protein (GFP)-expressing neural progenitor (NPCs) dissociated embryonic cords Fisher 344 rats. embedded spinal-cord-derived NPCs in glycol chitosan (gC)-oxidized hyaluronate (oHA) hydrogels (= NPC graft). graft was transplanted into spinal-cord-injured Sprague-Dawley Afterwards, mat positioned onto NPC-grafted region Injury + (INP) group). also added I) IN) groups as control groups. survival outcomes noticeably increased INP group compared those IN group. suggest separate implanting therapy for injuries.