CAPG interference induces apoptosis and ferroptosis in colorectal cancer cells through the P53 pathway

• DOI: 10.1016/j.mcp.2023.101919
• Publication Date: 2023-07-30T15:02:19Z
• Authors: Yingying Zhao; Rui Ma; Chuyue Wang; Rong Hu; Weili Wu; Xiang Sun; Baotao Chen; Wen Zhang; You Chen; Jiajian Zhou; Ping Yuan
• Keywords: P53; QH301-705.5; Microfilament Proteins; R; Nuclear Proteins; Apoptosis; Actins; 3. Good health; CAPG; CRC; Mice; Cell Line, Tumor; Medicine; Animals; Humans; Ferroptosis; Female; Biology (General); Tumor Suppressor Protein p53; Colorectal Neoplasms; Cell Proliferation

Given the high incidence and mortality rates of colorectal cancer (CRC) inadequacy existing treatments for many patients, this study aimed to explore potential Capping Actin Protein (CAPG), a protein involved in actin-related movements, as novel therapeutic target CRC.Bioinformatic analysis gene expression was conducted using UALCAN website. Cell proliferation measured CCK-8 kit. cycle, apoptosis, ferroptosis were analyzed flow cytometry. Tumorigenesis evaluated by subcutaneous inoculation CRC cells into BALB/c nude female mice. Differentially expressed genes signaling pathways identified RNA sequencing.CAPG significantly overexpressed human tissues its upregulation correlated with poor overall survival. CAPG knockdown led notable inhibition vitro vivo. Interference blocked cell cycle at G1 phase triggered apoptosis upregulating P53 pathway cells.CRC patients higher levels have poorer prognosis. inhibits ferroptosis, while promoting repressing pathway. Our suggests that may be prognosis treatment.