Transcription factor TEAD4 facilitates glycolysis and proliferation of gastric cancer cells by activating PKMYT1

• DOI: 10.1016/j.mcp.2023.101932
• Publication Date: 2023-11-02T00:06:58Z
• Authors: Lifen Zhan; Wen Wu; Qiongling Yang; Huiqun Shen; Limin Liu; Renzhi Kang
• Keywords: QH301-705.5; Proliferation; R; TEA Domain Transcription Factors; Membrane Proteins; Protein-Tyrosine Kinases; Protein Serine-Threonine Kinases; PKMYT1; 3. Good health; Stomach Neoplasms; Cell Line, Tumor; Medicine; Humans; TEAD4; Biology (General); Gastric cancer; Luciferases; Glycolysis; Transcription Factors; Cell Proliferation

Gastric cancer (GC) ranks third for deaths worldwide, and glycolysis is a hallmark of several cancers, including GC. TEAD4 plays role in establishing an oncogenic cascade Whether can influence the GC cells remains uncovered. Hence, this study attempted to investigate impact on by TEAD4.By using bioinformatics analysis, differentially expressed mRNAs were screened, downstream regulatory genes predicted. Expression levels PKMYT1 assessed qRT-PCR. The binding sites between predicted JASPAR database, meanwhile their modulatory relationship was confirmed through dual-luciferase assay chromatin Immunoprecipitation (ChIP). Cell viability proliferation assayed via CCK-8 colony formation assays. Glycolysis measured assaying extracellular acidification rate, oxygen consumption production pyruvic acid, lactate, citrate, malate. proteins (HK-2 PKM2) related Western blot.TEAD4 upregulated tissues cells. knockdown substantially repressed PKMYT1, target gene TEAD4, identified prediction, its expression elevated Dual-luciferase ChIP validated targeted promoter region TEAD4. As revealed rescue experiments, reversed stimulative effect cell forced PKMYT1.TEAD4 activated facilitate may be possible therapeutic targets