Biochemical Deconstruction and Reconstruction of Nuclear Matrix Reveals the Layers of Nuclear Organization

• DOI: 10.1016/j.mcpro.2023.100671
• Publication Date: 2023-10-19T04:13:34Z
• Authors: Ashish Bihani; Akshay K. Avvaru; Rakesh K. Mishra
• Keywords: Nuclear matrix; Nuclear pore; Proteome

Nuclear matrix (NuMat) is the fraction of eukaryotic nucleus insoluble to detergents and high-salt extractions that manifests as a pan-nuclear fiber-granule network. NuMat consists ribonucleoprotein complexes, members crucial nuclear functional modules, DNA fragments. Although captures organization nonchromatin space, very little known about components within NuMat. To understand components, we subfractionated it with increasing concentrations chaotrope guanidinium hydrochloride (GdnHCl) analyzed proteomic makeup fractions. We observe solubilization proteins at different GdnHCl finite independent broad biophysical properties protein sequences. Looking extraction pattern envelope pore complex, surmise this fractionation represents easily solubilized/loosely bound difficultly solubilized/tightly Microscopic analyses localization key across sequential in situ further elaborate on divergent patterns. Furthermore, solubilized 8M upon removal through dialysis, en masse renaturation leads RNA-dependent self-assembly fibrous structures. The major proteome component self-assembled fibers comes from solubilized, tightly component. This reveals organizational levels which may reflect structural architecture.