Into the Dark Serum Proteome: Personalized Features of IgG1 and IgA1 Repertoires in Severe COVID-19 Patients

• DOI: 10.1016/j.mcpro.2023.100690
• Publication Date: 2023-12-06T17:43:55Z
• Authors: Albert Bondt; Max Hoek; Kelly Dingess; Sem Tamara; Bastiaan de Graaf; Weiwei Peng; Maurits A. den Boer; Mirjam Damen; Ceri Zwart; Arjan Barendregt; Danique M.H. van ...; Douwe Schulte; Marloes Grobben; Khadija Tejjani; Jacqueline van Ri...; Franziska Völlmy; Joost Snijder; Francesca Fortini; Alberto Papi; Carlo Alberto Volta; Gianluca Campo; Marco Contoli; Marit J. van Gils; Savino Spadaro; Paola Rizzo; Albert J.R. Heck
• Keywords: Antibody Repertoire; Proteome; Polyclonal antibodies

Serum proteomics has matured and is now able to monitor hundreds of proteins quantitatively in large cohorts patients. However, the fine characteristics some most dominant serum, immunoglobulins, are these studies often ignored, due their vast, highly personalized, diversity sequences. Here, we focus exclusively on personalized features serum proteome distinctively chose study individual samples from a low population: elderly donors infected by severe acute respiratory syndrome corona virus 2 (SARS-CoV-2). By using mass spectrometry-based methods, immunoglobulin IgG1 IgA1 clonal repertoires were monitored longitudinally more than 50 obtained 17 Corona disease 2019 patients admitted intensive care units. These profiles used examine how each patient reacted SARS-CoV-2 infection. All revealed unique polyclonal substantial changes over time, with several new clones appearing following infection, few cases leading few, very high, abundant dominating repertoire. Several de novo sequenced through combinations top-down, middle-down, bottom-up approaches. This sequence line sequences deposited SARS-CoV-specific antibody database. In other patients, serological Ig treatment tocilizumab, that subsequently dominated Tocilizumab clearance could be monitored, half-life approximately 6 days was established. Overall, our longitudinal monitoring reveals responses traits patient, affected chosen clinical treatment. The impact observations argues for approach patients' diagnostics, both as well immune responses.