Dynamic Proteomic Changes in Tumor and Immune Organs Reveal Systemic Immune Response to Tumor Development

• DOI: 10.1016/j.mcpro.2024.100756
• Publication Date: 2024-03-28T18:05:31Z
• Authors: Zhike Li; Shuwen Liu; Zhouyong Gao; Linlin Ji; Jiaqi Jiao; Nairen Zheng; Xianju Li; Guangshun Wang; Jun Qin; Yi Wang
• Keywords: CCL18

In orthotopic mouse tumor models, progression is a complex process, involving interactions among cells, host cell-derived stromal and immune cells. Much attention has been focused on the its microenvironment, while host's macroenvironment including organs in response to tumorigenesis poorly understood. Here, we report temporal proteomic analysis subcutaneous three (LN, MLN, spleen) collected Days 0, 3, 7, 10, 14, 21 after inoculation of forestomach cancer cells syngeneic model. Bioinformatics identified key biological processes during distinct development phases, an initial acute response, attack by system, followed adaptive activation, build-up extracellular matrix. Proteomic changes LN spleen largely recapitulated dynamics tumor, consistent with defense D3, D10, evasion D21. contrast, MLN showed gradual sustained suggesting delayed from distal organ. Combined analyses tumors allowed identification potential therapeutic targets. A proof-of-concept experiment demonstrated that significant growth reduction can be achieved dual inhibition MEK DDR2. Together, our dataset provides useful resource for understanding interaction between system identifying new targets treatment.