Long non-coding RNA SNHG16 silencing inhibits proliferation and inflammation in Mycobacterium tuberculosis-infected macrophages by targeting miR-140-5p expression

• DOI: 10.1016/j.meegid.2022.105325
• Publication Date: 2022-06-29T06:21:11Z
• Authors: Wenna Sun; Xiushuang Zhang; Xiong He; Junxian Zhang; Xiaomeng Wang; Wen Lin; XiaoFeng Wang; Xueqiong Wu
• Keywords: Inflammation; 0301 basic medicine; MicroRNAs; 03 medical and health sciences; Macrophages; Humans; Tuberculosis; RNA, Long Noncoding; Mycobacterium tuberculosis; Biomarkers; Cell Proliferation; 3. Good health

The study investigated the clinical diagnostic value of long non-coding RNA (LncRNA) small nucleolar host gene 16 (SNHG16) and explored its underlying molecular mechanism through Mycobacterium tuberculosis (M. tuberculosiinfection macrophages.RT-qPCR analysis serum SNHG16 levels 66 healthy individuals, 67 latent TB (LTB) patients, active (ATB) patients. receiver-operating characteristic (ROC) curve to detect in In vitro, M. tuberculosis-infected macrophages, CCK-8 ELISA cell proliferation inflammatory factor levels. Luciferase reported assay was performed analyze targeting relationship between miR-140-5p.SNHG16 significantly elevated among them, ATB patients were higher than LTB ROC confirmed that could distinguish from controls, can be used as a good biomarker for TB. infection increased promoted inflammation macrophages. However, silencing reversed effect infection. miR-140-5p, direct target miRNA SNHG16, down-regulated negatively correlated with SNHG16. When miR-140-5p inhibited, alleviating on reversed.The present results suggested may new alleviate by inhibiting macrophages regulation miR-140-5p.