1,3,4-Thiadiazole derivatives of R-(+)-limonene benzaldehyde-thiosemicarbazones cause death in Trypanosoma cruzi through oxidative stress

• DOI: 10.1016/j.micinf.2016.07.007
• Publication Date: 2016-07-31T05:43:25Z
• Authors: Solange C. Martins; Danielle Lazarin-...; Vânia C. Desoti; Hugo Falzirolli; Cleuza C. da Silva; Tania Ueda-Nakamura; Sueli de O. Silva; Celso V. Nakamura
• Keywords: Benznidazole

This work evaluated the in vitro and vivo activity of TDZ 2 on Trypanosoma cruzi amastigotes determined possible mechanism action this compound T. death. inhibited proliferation had low haemolytic potential. It also induced morphological ultrastructural alterations. We observed a reduction cell volume, depolarization mitochondrial membrane, an increase ROS production, lipoperoxidation lipid bodies formation production nitric oxide, decrease reduced thiols levels and, presence autophagic vacuoles. The study found parasitemia animals treated with alone or combined benznidazole. Altogether, alterations by point to oxidative stress condition that lead