Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome
Proteomics
0301 basic medicine
0303 health sciences
Corynebacterium Infections
Proteome
Corynebacterium pseudotuberculosis
Microbiology
03 medical and health sciences
Infectious Diseases
Bacterial Proteins
Species Specificity
Lymphadenitis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Animals
Electrophoresis, Gel, Two-Dimensional
DOI:
10.1016/j.micpath.2013.05.004
Publication Date:
2013-05-16T05:31:15Z
AUTHORS (14)
ABSTRACT
This study involves the comparison between the exoproteomes of two different strains of Corynebacterium pseudotuberculosis, the etiologic agent of caseous lymphadenitis in small ruminants. In a previous study, based on a gel-free system (TPP-LC/MS(E)), 70 exoproteins for the strain 1002 and 67 for the strain C231, totaling 93 different extracellular proteins for C. pseudotuberculosis, were identified. In the present work, we have used 2D gel electrophoresis to resolve the extracellular proteins of both strains, which were then digested with trypsin, analyzed by MALDI-TOF/TOF and identified with the software MASCOT(®). A total of 45 extracellular proteins of C. pseudotuberculosis were identified by this approach. The comparative analysis between the strains 1002 and C231 identified 13 and 3 strain-specific proteins, respectively, 11 of which are novel. These newly identified proteins may play an important role in the physiology and virulence of C. pseudotuberculosis.
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