Phenotypic and functional features of innate and adaptive immunity as putative biomarkers for clinical status and leprosy reactions

• DOI: 10.1016/j.micpath.2018.09.011
• Publication Date: 2018-09-07T07:51:47Z
• Authors: Jairo Campos de C...; Marcelo Grossi Ar...; Jordana Grazziela...; Vanessa Peruhype-...; Cláudio Caetano A...; Marcela de Lima M...; Andréa Teixeira-C...; Olindo Assis Mart...; Márcio Sobreira S...
• Keywords: Ex vivo; Tuberculoid leprosy

The aim of this study was to identify phenotypic and functional biomarkers associated with distinct clinical status leprosy or reactions. included tuberculoid/borderline (BB/BT/T) lepromatous (BL/L) poles as well Type-1 Type-2 reactions along healthy controls (NI). A range peripheral blood innate (neutrophils - NEU monocytes MON) adaptive immunity (CD4+ CD8+ T-cells) were evaluated ex vivo upon in vitro stimuli M. leprae antigen. Data analysis allowed the selection NEUTLR4+ (ex vivo) CD4+IL-10+ (in vitro) universal increased all patients those exhibiting commonly found both patients, including decreased levels MONTGF-β+ MONTNF-α+, CD4+TGF-β+, CD8+TLR2+, CD8+TNF-α+, CD8+IL-4+ CD8+TGF-β+ vitro). Noteworthy that MONHLA-DR+ CD8+IL-10+ particularly BL/L patients. Leprosy reaction exhibited a larger list altered biomarkers, mainly involving activation markers (TLR2, TLR4, HLA-DR DAF-2T) MON CD4+ cells. In summary, provided insights about immunological features reactional episodes putative applicability, novel for complementary diagnosis future therapeutic approaches studies.