Characterization of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1-7-4 viruses) in northern China

• DOI: 10.1016/j.micpath.2019.103941
• Publication Date: 2019-12-17T19:43:44Z
• Authors: Changzhan Xie; Zhuo Ha; Fulong Nan; Ying Zhang; Han Zhang; Jinfeng Li; Ping Zhang; Jicheng Han; He Zhang; Xinyu Zhuang; Jinyong Zhang; Huijun Lu; Ningyi Jin
• Keywords: China; 0303 health sciences; Whole Genome Sequencing; Swine; Porcine Reproductive and Respiratory Syndrome; Genetic Variation; Genome, Viral; Disease Outbreaks; 3. Good health; 03 medical and health sciences; Animals; Porcine respiratory and reproductive syndrome virus; Lung; Phylogeny

The porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome (PRRS). Disease outbreaks caused by NADC30-like PRRSV strains were a bit prevalent in China in recent years. In the present study, two newly emerged PRRSV strains, which were designated as PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were found from piglets' lung tissues in Northern China. The virus belongs to lineage 1 of the PRRSV genotype 2 and is closely related to US strains that possess the open reading frame (ORF5) restriction fragment length polymorphism (RFLP) 1-7-4. The two strains were identified from infected weaning piglet herds in Zhaodong City, Heilongjiang province of China. The complete genome of the PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were 15093 nt and 15110 nt, and shared 96.7%-97.0% and 97.1%-97.4% similarities with the US identified, ISU10 and NADC34 strains respectively. Then the PRRSV-ZDXYL-China-2018-1 strain was successfully isolated from the clinical sample. Our results demonstrate, that the emergence of ORF5 RFLP 1-7-4-like PRRSVs in China, could pose a significant challenge to PRRSV epidemic prevention.