Efficacies of quorum sensing inhibitors, piericidin A and glucopiericidin A, produced by Streptomyces xanthocidicus KPP01532 for the control of potato soft rot caused by Erwinia carotovora subsp. atroseptica

• DOI: 10.1016/j.micres.2015.12.005
• Publication Date: 2015-12-21T23:33:30Z
• Authors: Ji Eun Kang; Jae Woo Han; Byeong Jun Jeon; Beom Seok Kim
• Keywords: 0301 basic medicine; Pyridines; Virulence Factors; Chromobacterium; Gene Expression Profiling; Quorum Sensing; Real-Time Polymerase Chain Reaction; Microbiology; Mass Spectrometry; Streptomyces; Anti-Bacterial Agents; 03 medical and health sciences; Aminoglycosides; Pectobacterium carotovorum; Pest Control, Biological; Plant Diseases; Solanum tuberosum

To discover potential inhibitors of the quorum sensing (QS) system, a library of microbial culture extracts was screened with Chromobacterium violaceumCV026 strain. The culture extract of Streptomyces xanthocidicus KPP01532 contained quorum-sensing inhibitors (QSIs) of the CV026 strain. The active constituents of the culture extract of strain KPP01532 were purified using a series of chromatographic procedures, and based on data from NMR and mass spectroscopy, piericidin A and glucopiericidin A were identified. Erwinia carotovora subsp. atroseptica (Eca) is a plant pathogen that causes blackleg and soft rot diseases on potato stems and tubers. The virulence factors of Eca are regulated by QS. The expression of virulence genes (pelC, pehA, celV and nip) under the control of QS was monitored using quantitative real-time PCR (qRT-PCR). The transcription levels of the four genes were significantly lower when Eca was exposed to piericidin A or glucopiericidin A. These two compounds displayed similar control efficacies against soft rot caused by Eca in potato slices as furanone C-30. Therefore, piericidin A and glucopiericidin A are potential QSIs that suppress the expression of the virulence genes of Eca, suggesting that they could have potential use as control agents of soft rot disease on potato tubers.