The Gα1-cAMP signaling pathway controls conidiation, development and secondary metabolism in the taxol-producing fungus Pestalotiopsis microspora

• DOI: 10.1016/j.micres.2017.06.003
• Publication Date: 2017-06-16T06:46:07Z
• Authors: Xi Yu; Heng Liu; Xueliang Niu; Oren Akhberdi; Dongsheng Wei; Dan Wang; Xudong Zhu
• Keywords: 0303 health sciences; Paclitaxel; Genes, Fungal; Spores, Fungal; GTP-Binding Protein alpha Subunits; Histone Deacetylases; 03 medical and health sciences; Gene Expression Regulation, Fungal; Saccharomycetales; Cyclic AMP; Polyketide Synthases; Signal Transduction

G-protein-mediated signaling pathways regulate fungal morphogenesis, development and secondary metabolism. In this study, we report a gene, pgα1, that putatively encodes the α-subunit of a group I G protein in Pestalotiopsis microspora NK17, which is known to produce various secondary metabolites, including the antitumor drug taxol and pestalotiollide B (PB). Mutants of pgα1 showed retarded vegetative growth, aging of the mycelium, premature conidiation, deformed conidia, significantly increased melanin production, and a sharp decrease in PB production. The introduction of extra copies of pgα1 led to a different phenotype that was characterized by enhanced production of PB. qRT-PCR revealed that the expression of pks1, which encodes melanin polyketide synthase, an enzyme that is involved in 1, 8-dihydroxynaphthalene (DHN) melanin biosynthesis, was up regulated by 55-fold in the absence of pgα1. Changes in conidiation and PB production in pgα1 mutants were able to be restored by the addition of exogenous cAMP. The deficiencies of PB production and conidiation in Δpgα1 were not able to be rescued by deletion or overexpression of a previously reported histone deacetylase gene (hid1), suggesting that pgα1 is able to override the effect of hid1 on PB production and conidiation. Our results suggested that the G protein-cAMP pathway plays a critical role in vegetative growth as well as in asexual development of P. microspora.