Accurate and sensitive analysis of Staphylococcus aureus through CRISPR-Cas12a based recycling signal amplification cascades for early diagnosis of skin and soft tissue infections

• DOI: 10.1016/j.mimet.2021.106167
• Publication Date: 2021-02-10T16:53:02Z
• Authors: JingJing Wei
• Keywords: 0301 basic medicine; Staphylococcus aureus; 03 medical and health sciences; Soft Tissue Infections; Humans; Staphylococcal Skin Infections; CRISPR-Cas Systems; Staphylococcal Infections; Nucleic Acid Amplification Techniques; Sensitivity and Specificity; 12. Responsible consumption; 3. Good health

Early-diagnosis and treatments of skin and soft tissue infections remain a huge challenge due to the difficulties in the detection of trace amounts of bacteria. We develop here a novel method through CRISPR-Cas12a based recycling signal amplification cascades and demonstrated its feasibility of Staphylococcus aureus detection in a sensitive and accurate way. The highlights of the proposed method are calculated as: i) the designed allosteric probe is responsible for accurate identification of SA through PBP2a-specific aptamer; ii) high sensitivity from three processes, including DNA polymerase-based target SA release, Nb.BbvCI enzyme induced ssDNA generation and attached CRISPR-Cas12a. As a result, the proposed method exhibited a detection range from 106 to 102 CFU/ml. Eventually, we believe that the proposed method could be expanded for the construction of diverse sensing platforms for detecting different trace bacteria.