Tyrphostin A9 improves blastocyst development in porcine embryos through induction of dynamin-related protein 1-dependent mitochondrial fission

• DOI: 10.1016/j.mito.2017.05.008
• Publication Date: 2017-05-29T04:03:02Z
• Authors: Jae-Hyun Ahn; Hyo-Jin Park; Jin-Woo Kim; Jae-Young Park; Soo-Yong Park; Seul-Gi Yang; Chang-Hyun Kim; Seung-Bin Yoon; Sun-Uk Kim; Kyu-Tae Chang; Deog-Bon Koo
• Keywords: Dynamins; 0303 health sciences; 03 medical and health sciences; Blastocyst; Swine; Animals; Female; Tyrphostins; Mitochondrial Dynamics

Mitochondrial dynamics are associated with the development of porcine embryos. However, little is known about the effects of mitochondrial dynamics-related genes (Drp1 and pDrp1-Ser616) on early porcine embryo development. Here, we investigated the effect of Drp1-dependent mitochondrial fission signaling on the development of porcine embryos using the mitochondrial fission inducer, tyrphostin A9 (TA9). We determined that TA9 (1μM) treated embryos were increased the mitochondrial functions, blastocyst development rate and quality, as well as decreased mitochondria-specific superoxide and mitochondrial apoptosis. Thus, TA9-induced appropriate mitochondrial fission improved the developmental competence via maintenance of a balance in mitochondrial dynamics in porcine embryo.