Human Asf1 Regulates the Flow of S Phase Histones during Replicational Stress

• DOI: 10.1016/j.molcel.2004.12.018
• Publication Date: 2005-01-25T19:49:35Z
• Authors: Anja Groth; Dominique Ray-Gallet; Jean-Pierre Quivy; Jiri Lukas; Jiri Bartek; Geneviève Almouzni
• Keywords: DNA Replication; 0301 basic medicine; 0303 health sciences; Macromolecular Substances; Cell Cycle Proteins; Cell Biology; Cell Fractionation; Chromatin; S Phase; Histones; 03 medical and health sciences; Cell Line, Tumor; Animals; Humans; Hydroxyurea; Molecular Biology; Molecular Chaperones; Nucleic Acid Synthesis Inhibitors

Maintenance of chromosomal integrity requires tight coordination of histone biosynthesis with DNA replication. Here, we show that extracts from human cells exposed to replication stress display an increased capacity to support replication-coupled chromatin assembly. While in unperturbed S phase, hAsf1 existed in equilibrium between an active form and an inactive histone-free pool, replication stress mobilized the majority of hAsf1 into an active multichaperone complex together with histones. This active multichaperone complex was limiting for chromatin assembly in S phase extracts, and hAsf1 was required for the enhanced assembly activity in cells exposed to replication stress. Consistently, siRNA-mediated knockdown of hAsf1 impaired the kinetics of S phase progression. Together, these data suggest that hAsf1 provides the cells with a buffering system for histone excess generated in response to stalled replication and explains how mammalian cells maintain a critical "active" histone pool available for deposition during recovery from replication stresses.