Ring1B Compacts Chromatin Structure and Represses Gene Expression Independent of Histone Ubiquitination

• DOI: 10.1016/j.molcel.2010.02.032
• Publication Date: 2010-05-14T08:41:38Z
• Authors: Ragnhild Eskeland; Martin Leeb; Graeme R. Grimes; Clémence Kress; Shelagh Boyle; Duncan Sproul; Nick Gilbert; Yuhong Fan; Arthur I. Skoultchi; Anton Wutz; Wendy A. Bickmore
• Keywords: /dk/atira/pure/subjectarea/asjc/1300/1312; Transcription, Genetic; Ubiquitin-Protein Ligases; Down-Regulation; Polycomb-Group Proteins; DEVBIO; Methylation; Cell Line; Histones; Mice; Animals; Molecular Biology; Embryonic Stem Cells; Homeodomain Proteins; Polycomb Repressive Complex 1; Polycomb Repressive Complex 2; Ubiquitination; Acetylation; Cell Differentiation; DNA; Cell Biology; Chromatin Assembly and Disassembly; Repressor Proteins; Mutation; /dk/atira/pure/subjectarea/asjc/1300/1307; Protein Processing, Post-Translational

How polycomb group proteins repress gene expression in vivo is not known. While histone-modifying activities of the polycomb repressive complexes (PRCs) have been studied extensively, in vitro data have suggested a direct activity of the PRC1 complex in compacting chromatin. Here, we investigate higher-order chromatin compaction of polycomb targets in vivo. We show that PRCs are required to maintain a compact chromatin state at Hox loci in embryonic stem cells (ESCs). There is specific decompaction in the absence of PRC2 or PRC1. This is due to a PRC1-like complex, since decompaction occurs in Ring1B null cells that still have PRC2-mediated H3K27 methylation. Moreover, we show that the ability of Ring1B to restore a compact chromatin state and to repress Hox gene expression is not dependent on its histone ubiquitination activity. We suggest that Ring1B-mediated chromatin compaction acts to directly limit transcription in vivo.