Control of Cdc28 CDK1 by a Stress-Induced lncRNA

• DOI: 10.1016/j.molcel.2014.01.006
• Publication Date: 2014-02-06T17:37:40Z
• Authors: Mariona Nadal-Rib...; Carme Solé; Zhenyu Xu; Lars M. Steinmetz; Eulàlia de Nadal; Francesc Posas
• Keywords: 0301 basic medicine; Chromatin Immunoprecipitation; Saccharomyces cerevisiae Proteins; Transcription, Genetic; Green Fluorescent Proteins; Saccharomyces cerevisiae; 03 medical and health sciences; Transcripció genètica; Osmotic Pressure; Gene Expression Regulation, Fungal; Mitogen-Activated Protein Kinase 8; Promoter Regions, Genetic; Molecular Biology; 3' Untranslated Regions; Cell Cycle; Proteïnes quinases -- Metabolisme; Cell Biology; Oligonucleotides, Antisense; Flow Cytometry; Nucleosomes; Saccharomyces cerevisiae -- Metabolisme; RNA, Long Noncoding; Mitogen-Activated Protein Kinases; CDC28 Protein Kinase, S cerevisiae

Genomic analysis has revealed the existence of a large number of long noncoding RNAs (lncRNAs) with different functions in a variety of organisms, including yeast. Cells display dramatic changes of gene expression upon environmental changes. Upon osmostress, hundreds of stress-responsive genes are induced by the stress-activated protein kinase (SAPK) p38/Hog1. Using whole-genome tiling arrays, we found that Hog1 induces a set of lncRNAs upon stress. One of the genes expressing a Hog1-dependent lncRNA in antisense orientation is CDC28, the cyclin-dependent kinase 1 (CDK1) that controls the cell cycle in yeast. Cdc28 lncRNA mediates the establishment of gene looping and the relocalization of Hog1 and RSC from the 3' UTR to the +1 nucleosome to induce CDC28 expression. The increase in the levels of Cdc28 results in cells able to reenter the cell cycle more efficiently after stress. This may represent a general mechanism to prime expression of genes needed after stresses are alleviated.