EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation

• DOI: 10.1016/j.molcel.2018.03.018
• Publication Date: 2018-04-19T15:42:51Z
• Authors: Jong-Ho Lee; Rui Liu; Jing Li; Yugang Wang; Lin Tan; Xin-Jian Li; Xu Qian; Chuanbao Zhang; Yan Xia; Daqian Xu; Wei Guo; Zhiyong Ding; Linyong Du; Yanhua Zheng; Qianming Chen; Philip L. Lorenzi; Gordon B. Mills; Tao Jiang; Zhimin Lu
• Keywords: Feedback, Physiological; Male; Glucose Transporter Type 1; Mice, Inbred BALB C; 0303 health sciences; Brain Neoplasms; Mice, Nude; Acetylation; Phosphofructokinase-1, Type C; Lysine Acetyltransferase 5; Class Ia Phosphatidylinositol 3-Kinase; Enzyme Activation; ErbB Receptors; Phosphatidylinositol 3-Kinases; 03 medical and health sciences; Cell Line, Tumor; Fructosediphosphates; Animals; Humans; Glioblastoma; Glycolysis; Adaptor Proteins, Signal Transducing

EGFR activates phosphatidylinositide 3-kinase (PI3K), but the mechanism underlying this activation is not completely understood. We demonstrated here that EGFR activation resulted in lysine acetyltransferase 5 (KAT5)-mediated K395 acetylation of the platelet isoform of phosphofructokinase 1 (PFKP) and subsequent translocation of PFKP to the plasma membrane, where the PFKP was phosphorylated at Y64 by EGFR. Phosphorylated PFKP binds to the N-terminal SH2 domain of p85α, which is distinct from binding of Gab1 to the C-terminal SH2 domain of p85α, and recruited p85α to the plasma membrane resulting in PI3K activation. PI3K-dependent AKT activation results in enhanced phosphofructokinase 2 (PFK2) phosphorylation and production of fructose-2,6-bisphosphate, which in turn promotes PFK1 activation. PFKP Y64 phosphorylation-enhanced PI3K/AKT-dependent PFK1 activation and GLUT1 expression promoted the Warburg effect, tumor cell proliferation, and brain tumorigenesis. These findings underscore the instrumental role of PFKP in PI3K activation and enhanced glycolysis through PI3K/AKT-dependent positive-feedback regulation.