The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage

• DOI: 10.1016/j.molcel.2018.09.010
• Publication Date: 2018-10-18T14:49:54Z
• Authors: Sandra Piquet; Florent Le Parc; Siau-Kun Bai; Odile Chevallier; Salomé Adam; Sophie E. Polo
• Keywords: Histone code; Histone-modifying enzymes

Safeguarding cell function and identity following a genotoxic stress challenge entails tight coordination of DNA damage signaling repair with chromatin maintenance. How this is achieved what impact on integrity remains elusive. Here, we address these questions by investigating the mechanisms governing distribution in mammalian histone variant H2A.X, central player signaling. We reveal that H2A.X deposited de novo at sites repair-coupled manner, whereas H2A.Z evicted, thus reshaping landscape sites. Our mechanistic studies further identify chaperone FACT (facilitates transcription) as responsible for deposition newly synthesized H2A.X. Functionally, demonstrate potentiates H2A.X-dependent damage. propose new reflects experience may help tailor to progression.