System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection

• DOI: 10.1016/j.molcel.2019.01.017
• Publication Date: 2019-02-22T04:53:26Z
• Authors: Manuel Garcia-Moreno; Marko Noerenberg; Shuai Ni; Aino I. Järvelin; Esther González-A...; Caroline E. Lenz; Marcel Bach-Pages; Victoria Cox; Rosario Avolio; Thomas Davis; Svenja Hester; Thibault J.M. Sohier; Bingnan Li; Gregory Heikel; Gracjan Michlewski; Miguel A. Sanz; Luis Carrasco; Emiliano P. Ricci; Vicent Pelechano; Ilan Davis; Bernd Fischer; Shabaz Mohammed; Alfredo Castello
• Keywords: Profiling (computer programming)

Highlights•A quarter of the RBPome changes upon SINV infection•Alterations in RBP activity are largely explained by RNA availability•Altered RBPs crucial for viral infection efficacy•GEMIN5 binds to 5′ end RNAs and regulates gene expressionSummaryThe compendium RNA-binding proteins (RBPs) has been greatly expanded development RNA-interactome capture (RIC). However, it remained unknown if complement response environmental perturbations whether these rearrangements important. To answer questions, we developed "comparative RIC" applied cells challenged with an virus called sindbis (SINV). Over 200 display differential interaction infection. These alterations mainly driven loss cellular mRNAs emergence RNA. stimulated redistribute replication factories regulate capacity infect. For example, ablation XRN1 causes be refractory SINV, while GEMIN5 moonlights as a regulator expression. In summary, availability controls localization function SINV-infected cells.Graphical abstract