Collisions of RNA polymerases behind the replication fork promote alternative RNA splicing in newly replicated chromatin

• DOI: 10.1016/j.molcel.2023.11.036
• Publication Date: 2023-12-26T15:38:55Z
• Authors: Federica Bruno; Cristóbal Coronel...; Cristina González...
• Keywords: RNA polymerase II; Transcription

DNA replication produces a global disorganization of chromatin structure that takes hours to be restored. However, how these rearrangements affect the regulation gene expression and maintenance cell identity is not clear. Here, we use ChOR-seq ChrRNA-seq experiments analyze RNA polymerase II (RNAPII) activity nascent synthesis during first after in human cells. We observe transcription elongation rapidly reactivated but RNAPII abundance distribution are altered, producing heterogeneous changes synthesis. Moreover, this wave results blockages behind fork, leading alternative splicing. Altogether, our deepen understanding transcriptional programs regulated division uncover molecular mechanisms explain why an important source variability.