Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells
0301 basic medicine
EXOPOLYSACCHARIDES
Swine
Sus scrofa
ANTI-INFLAMMATORY ACTIVITY
03 medical and health sciences
https://purl.org/becyt/ford/3.1
Animals
https://purl.org/becyt/ford/3
Intestinal Mucosa
Cells, Cultured
Swine Diseases
Lactobacillus delbrueckii
0303 health sciences
PORCINE INTESTINAL EPITHELIAL CELLS
Polysaccharides, Bacterial
ANTIVIRAL ACTIVITY
Epithelial Cells
Interferon-beta
PROBIOTICS
Immunity, Innate
Toll-Like Receptor 2
Toll-Like Receptor 3
3. Good health
Toll-Like Receptor 4
Poly I-C
Gene Expression Regulation
Virus Diseases
IMMUNE MODULATION
Cytokines
Signal Transduction
DOI:
10.1016/j.molimm.2017.07.009
Publication Date:
2017-08-08T19:15:24Z
AUTHORS (14)
ABSTRACT
Previous studies demonstrated that the extracellular polysaccharides (EPSs) produced by Lactobacillus delbrueckii OLL1073R-1 (LDR-1) improve antiviral immunity, especially in the systemic and respiratory compartments. However, it was not studied before whether those EPSs are able to beneficially modulate intestinal antiviral immunity. In addition, LDR-1-host interaction has been evaluated mainly with immune cells while its interaction with intestinal epithelial cells (IECs) was not addressed before. In this work, we investigated the capacity of EPSs from LDR-1 to modulate the response of porcine IECs (PIE cells) to the stimulation with the Toll-like receptor (TLR)-3 agonist poly(I:C) and the role of TLR2, TLR4, and TLR negative regulators in the immunoregulatory effect. We showed that innate immune response triggered by TLR3 activation in porcine IECs was differentially modulated by EPS from LDR-1. EPSs treatment induced an increment in the expression of interferon (IFN)-α and IFN-β in PIE cells after the stimulation with poly(I:C) as well as the expression of the antiviral factors MxA and RNase L. Those effects were related to the reduced expression of A20 in EPS-treated PIE cells. EPS from LDR-1 was also able to reduce the expression of IL-6 and proinflammatory chemokines. Although further in vivo studies are needed, our results suggest that these EPSs or a yogurt fermented with LDR-1 have potential to improve intestinal innate antiviral response and protect against intestinal viruses.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (33)
CITATIONS (81)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....