Metabolic and transcriptional response to a high-fat diet in Drosophila melanogaster

• DOI: 10.1016/j.molmet.2013.10.003
• Publication Date: 2013-10-24T01:31:51Z
• Authors: Erilynn T. Heinri...; Hui Zhang; James E. Robinson; John Ngo; Soda Diop; Rolf Bodmer; William J. Joiner; Christian M. Metallo; Gabriel G. Haddad
• Keywords: Fatty Acid Metabolism; Carbohydrate Metabolism; Metabolic pathway

Obesity has dramatically increased in prevalence, making it essential to understand its accompanying metabolic changes. Modeling diet-induced obesity Drosophila melanogaster (fruit flies), we elucidated transcriptional and changes w (1118) flies on a high-fat diet (HFD). Mass spectrometry-based metabolomics revealed altered fatty acid, amino carbohydrate metabolism with HFD. Microarray analysis uncovered nitrogen metabolism, including CG9510, homolog of human argininosuccinate lyase (ASL). CG9510 knockdown phenocopied traits observed HFD, namely triglyceride levels decreased cold tolerance. Restoration expression ameliorated negative consequences Metabolomic confirmed functional similarity ASL, regulating the balance carbon metabolism. In summary, found that HFD suppresses expression, gene required for proper storage stress These results draw an important link between regulation acid response obesity.