Patient‐derived xenografts recapitulate molecular features of human uveal melanomas

• DOI: 10.1016/j.molonc.2013.02.004
• Publication Date: 2013-02-26T05:46:51Z
• Authors: Cécile Laurent; David Gentien; Sophie Piperno-Ne...; Fariba Némati; André Nicolas; Bruno Tesson; Laurence Desjardins; Pascale Mariani; Audrey Rapinat; Xavier Sastre-Garau; Jérôme Couturier; Philippe Hupé; Leanne de Koning; Thierry Dubois; Sergio Roman-Roman; Marc-Henri Stern; Emmanuel Barillot; J. William Harbour; Simon Saule; Didier Decaudin
• Keywords: Proto-Oncogene Proteins B-raf; Uveal Neoplasms; MAP Kinase Signaling System; Tumor Suppressor Proteins; DNA Mutational Analysis; GTP-Binding Protein alpha Subunits; Gene Expression Regulation, Neoplastic; Mice; 03 medical and health sciences; 0302 clinical medicine; Mutation; Tumor Cells, Cultured; Animals; GTP-Binding Protein alpha Subunits, Gq-G11; Heterografts; Humans; Transcriptome; Melanoma; Ubiquitin Thiolesterase; Oligonucleotide Array Sequence Analysis

We have previously developed a new method for the development and maintenance of uveal melanoma (UM) xenografts in immunodeficient mice. Here, we compare genetic profiles primary tumors to their corresponding that been passaged over time. The study included sixteen UMs at very early (P1), (P4), late (P9) vivo passages. were analyzed mutation status GNAQ, GNA11, GNAS, GNA15, BAP1, BRAF, chromosomal copy number alterations using Affymetrix GeneChip® Genome‐Wide Human SNP6.0 arrays, gene expression Exon 1.0 ST BAP1 mRNA protein expression, MAPK pathway Reverse Phase Protein Arrays (RPPA). UM accurately recapitulated features human they exhibited stability course maintenance. Our technique establishing maintaining as xenograft mice exhibit high degree conservation between multiple passages vivo. These models therefore constitute valuable preclinical tool drug screening UM.