Hierarchical self-assembly of heparin-PEG end-capped porous silica as a redox sensitive nanocarrier for doxorubicin delivery

• DOI: 10.1016/j.msec.2016.04.085
• Publication Date: 2016-04-30T14:21:20Z
• Authors: Thu Thao Nguyen Thi; Tuong Vi Tran; Ngoc Quyen Tran; Cuu Khoa Nguyen; Dai Hai Nguyen
• Keywords: Drug Carriers; Cell Death; Heparin; Proton Magnetic Resonance Spectroscopy; Temperature; Silicon Dioxide; 01 natural sciences; Polyethylene Glycols; 0104 chemical sciences; 3. Good health; Drug Liberation; Drug Delivery Systems; X-Ray Diffraction; Doxorubicin; Spectroscopy, Fourier Transform Infrared; Humans; Nanoparticles; Oxidation-Reduction; HeLa Cells

Porous nanosilica (PNS) has been attracting a great attention in fabrication carriers for drug delivery system (DDS). However, unmodified PNS-based carriers exhibited the initial burst release of loaded bioactive molecules, which may limit their potential clinical application. In this study, the surface of PNS was conjugated with adamantylamine (A) via disulfide bonds (PNS-SS-A) which was functionalized with cyclodextrin-heparin-polyethylene glycol (CD-HPEG) for redox triggered doxorubicin (DOX) delivery. The modified PNS was successfully formed with spherical shape and diameter around 50nm determined by transmission electron microscopy (TEM). DOX was efficiently trapped in the PNS-SS-A@CD-HPEG and slowly released in phosphate buffered saline (PBS) without any initial burst effect. Importantly, the release of DOX was triggered due to the cleavage of the disulfide bonds in the presence of dithiothreitol (DTT). In addition, the MTT assay data showed that PNS-SS-A@CD-HPEG was a biocompatible nanocarrier and reduced the toxicity of DOX. These results demonstrated that PNS-SS-A@CD-HPEG has great potential as a novel nanocarrier for anticancer drug in cancer therapy.