Controlled delivery of bone morphogenic protein-2-related peptide from mineralised extracellular matrix-based scaffold induces bone regeneration

• DOI: 10.1016/j.msec.2021.112182
• Publication Date: 2021-05-13T02:29:42Z
• Authors: Chunqing Meng; Weijie Su; Man Liu; Sheng Yao; Qiuyue Ding; Keda Yu; Zekang Xiong; Kaifang Chen; Xiaodong Guo; Lin Bo; Tingfang Sun
• Keywords: Matrix (chemical analysis)

Ideal bone tissue engineering scaffolds composed of extracellular matrix (ECM) require excellent osteoconductive ability to imitate the environment. We developed a mineralised tissue-derived ECM-modified true ceramic (TBC) scaffold for delivery aspartic acid-modified morphogenic protein-2 (BMP-2) peptide (P28) and assessed its osteogenic capacity. Decellularized ECM from porcine small intestinal submucosa (SIS) was coated onto surface TBC, followed by mineralisation modification (mSIS/TBC). P28 subsequently immobilised in absence crosslinker. The alkaline phosphatase activity other differentiation marker results showed that osteogenesis P28/mSIS/TBC significantly greater than TBC mSIS/TBC groups. In addition, examine capability this system vivo, we established rat calvarial defect model evaluated new area blood vessel density. Histological observation exhibited favourable regeneration efficacy. This study proposes use loaded with as promising applications.