Extracellular matrix-inspired surface coatings functionalized with dexamethasone-loaded liposomes to induce osteo- and chondrogenic differentiation of multipotent stem cells

• DOI: 10.1016/j.msec.2021.112516
• Publication Date: 2021-10-29T02:26:25Z
• Authors: Yazmin A. Brito B...; Catharina Husteden; Jumanah Alherz; Bodo Fuhrmann; Christian Wölk; Thomas Groth
• Keywords: Chondrogenesis; Aggrecan

Biomimetic surface coatings can be combined with conventional implants to mimic the extracellular matrix (ECM) of surrounding tissue make them more biocompatible. Layer-by-layer technique (LbL) used for making by alternating adsorption polyanions and polycations from aqueous solutions without need chemical reactions. Here, polyelectrolyte multilayer (PEM) systems is made hyaluronic acid (HA) as polyanion Collagen I (Col) polycation ECM connective tissue. The PEM are dexamethasone (Dex)-loaded liposomes achieve a local delivery protection this drug stimulation osteo- chondrogenic differentiation multipotent stem cells. possess positive charge that required immobilization on PEM. properties system show zeta potential after liposome decrease in wettability, both promoting cell adhesion spreading C3H10T1/2 embryonic mouse fibroblasts. Differentiation was prominent embedded Dex-loaded compared basal use free supernatant. This evident immunohistochemical staining an upregulation expression genes, which play key role osteogenesis (RunX2, ALP, Osteocalcin (OCN)) chondrogenesis (Sox9, aggrecan (ACAN), collagen type II), determined quantitative Real-time polymerase chain reaction (qRT-PCR) 21 days. These findings indicate designed liposome-loaded have high implant induce bone cartilage needed example osteochondral implants.