Construction of 3D-Bioprinted cartilage-mimicking substitute based on photo-crosslinkable Wharton's jelly bioinks for full-thickness articular cartilage defect repair

• DOI: 10.1016/j.mtbio.2023.100695
• Publication Date: 2023-06-09T06:41:56Z
• Authors: Guanhuai Hu; Zhuo Liang; Zhenlin Fan; Mengyuan Yu; Qingqing Pan; Yan Nan; Wei Zhang; Lei Wang; Xiansong Wang; Yujie Hua; Guangdong Zhou; Wenjie Ren
• Keywords: Chondrogenesis; Articular cartilage repair; 3D bioprinting; Gelatin; Biocompatibility; Wharton's jelly

Three-dimensional (3D) bioprinted cartilage-mimicking substitutes for full-thickness articular cartilage defect repair have emerged as alternatives to in situ models. However, there has been very limited breakthrough regeneration based on 3D bioprinting owing the lack of ideal bioinks with printability, biocompatibility, bioactivity, and suitable physicochemical properties. In contrast animal-derived natural polymers or acellular matrices, human-derived Wharton's jelly is biocompatible hypoimmunogenic an abundant source. Although can mimic chondrogenic microenvironment, it remains challenging prepare both printable biologically active from this material. Here, we firstly prepared methacryloyl-modified (AWJMA) using a previously established photo-crosslinking strategy. Subsequently, combined gelatin AWJMA obtain hybrid hydrogel that exhibited properties biological activities were bioprinting. Moreover, bone marrow mesenchymal stem cell-loaded 3D-bioprinted had superior advantages survival, proliferation, spreading, differentiation cells, which enabled satisfactory model rabbit knee joint. The current study provides novel strategy repair.