Spatial transcriptomic interrogation of the tumour-stroma boundary in a 3D engineered model of ameloblastoma

• DOI: 10.1016/j.mtbio.2023.100923
• Publication Date: 2023-12-21T18:48:08Z
• Authors: Deniz Bakkalci; Georgina Al-Badri; Wei Yang; Andy Nam; Yan Liang; Syed Ali Khurram; Susan Heavey; Stefano Fedele; Umber Cheema
• Keywords: Adamantinoma; Compartment (ship)

Stromal cells are key components of the tumour microenvironment (TME) and their incorporation into 3D engineered tumour-stroma models is essential for mimicry. By engineering tumouroids with distinct stromal compartments, it has been possible to identify how gene expression altered influenced by presence different cells. Ameloblastoma a benign epithelial jawbone. In engineered, multi-compartment spatial transcriptomics revealed an upregulation oncogenes in ameloblastoma transcriptome where osteoblasts were present compartment (bone stroma). Where gingival fibroblast stroma was increased matrix remodelling genes. This study provides evidence show stromal-specific effect on behaviour illustrates importance biologically relevant models. Our novel results that causes genes which directly correlates measured invasion model. contrast bone increases