Bcl-2 supports survival and metabolic fitness of quiescent tissue-resident ILC3

• DOI: 10.1016/j.mucimm.2023.07.001
• Publication Date: 2023-07-29T19:14:29Z
• Authors: James I. King; Felipe Melo-Gonzalez; Bert Malengier-De...; Roser Tachó-Piñot; Marlene S Magalhaes; Suzanne H. Hodge; Xavier Romero Ros; Rebecca Gentek; Matthew R. Hepworth
• Keywords: Innate lymphoid cell

Group 3 innate lymphoid cells (ILC3) are potent effector with critical roles in enforcing immunity, barrier integrity and tissue homeostasis along the gastrointestinal tract. ILC3 considered primarily tissue-resident cells, seeding tract during embryonic stages early life. However, mechanisms through which mature maintained within adult tissues poorly understood. Here, we report that tissue-inducer-like (LTi-like) exhibit minimal turnover healthy intestinal tract, persist for extended periods of time, display a quiescent phenotype. Strikingly, enteric bacterial infection LTi-like also negligible hematopoietic replenishment remain non-proliferative, despite robustly producing cytokines. Survival was found to be dependent upon balance between metabolic activity required drive function anti-apoptotic programs. Notably, pro-survival protein B-cell lymphoma-2 (Bcl-2) survival ex vivo but rendered partially dispensable if mitochondrial respiration inhibited. Together demonstrate tissue-resident, population independently survive microenvironment.